2020-04-03
Likes

A research paper entitled Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction was published in Nature Communications by Professor Xing Da's team of the College of Biophotonics, the MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, Guangdong Provincial Key Laboratory. This article presents a detection system (called ‘APC-Cas’) that can detect very low numbers of a bacterial pathogen without isolation, using a three-stage amplification to generate powerful fluorescence signals. APC-Cas involves a combination of nucleic acid-based allosteric probes and CRISPR-Cas13a components.

202002267bcb79.jpg

The research team used aptamers to construct an allosteric DNA molecule that can bind to a target pathogen and convert it into its active configuration. In its active configuration, the catalytic pathogen binds to aptamers (primary amplification), triggers isothermal amplification (secondary amplification), and activates the trans-cutting activity CRISPR / Cas13a (tertiary amplification), which amplifies the fluorescent signal by cutting RNA report probes, the cascade three-stage amplification produces detectable fluorescence signals, enabling direct detection of pathogens without the needs for isolation or extraction of nucleic acids. In addition, the team demonstrated greater sensitivity, accuracy, and ease of operation with APC-Cas compared to traditional real-time PCR methods through methodological comparisons, it indicates that APC-Cas has potential clinical value in the diagnosis of bacterial diseases.

20200226cb9871.jpg

The team has been working in recent years to develop CRISPR technology for Molecular Diagnostics, by designing functional molecules of nucleic acids that can be used to identify and detect tumor-related MicroRNAs and pathogenic microbes, combining the specific recognition ability of CRISPR proteins, a variety of CRISPR molecular diagnostic techniques have been developed to provide technical support for early detection of tumors and rapid detection of pathogens.

The lead author is Shen Jinjin, a postgraduate student at the College of Biophotonics of South China Normal University, and professors Hu Jiaming and Xing Da are co-corresponding authors of the paper. This work has been supported by the National Natural Science Foundation of China and the Natural Science Foundation of Guangdong Province.

Paper Link:

https://www.nature.com/articles/s41467-019-14135-9


Translated by Li Xinyi

Proofread by Edwin Baak

Reviewed by Li Jianru

 

What to read next:

Associate Professor Dr. Fei Jifeng of the SCNU Institute for Brain Research and Rehabilitation Published a Paper in Nature
On January 24, Dr Fei Jifeng, Distinguished Young Talent of the Institute for Brain Research and Rehabilitation, South China Normal University and researchers from dozen scientific research institutes like Max Planck Institute of Molecular Cell Bi
Prof. Li Sheng and His Team Published Important Research Findings in Nature Communications
On March 20, the research paper titled “The genomic and functional landscapes of developmental plasticity in the American cockroach”, jointly written by Prof Li Sheng and his team from School of Life Sciences and Institute of Insect Science and Techn
SCNU Undergraduates of School of Chemistry and Environment Published Important Findings in《Angew. Chem. Int. Ed.》
Recently, members of the Innovation and Entrepreneurship Training Program of School of Chemistry and Environment of
New breakthrough in Video Electronic Paper in China
From May 21-25, 2018, the latest accomplishments in color video electronic paper displays manufactured by the team
Professor Xiang Hui Published Important Findings in Nature Ecology and Evolution
The research paper titled “The evolutionary road from wild moth to domestic silkworm” by Professor Xiang Hui fr
The Latest Findings from the Team of Professor Ying Guangguo of the Environmental Research Institute Published in "Environmental Science & Technology"
Dr Shi Wenjun, team member with Professor Ying Guangguo at the Environmental Research Institute of South China N
The Research Group of Researcher Jiang Feng of the SCNU Institute of Optoelectronic Materials and Technology Published Important Findings in the ACS Journal
Recently, the "Young Talent " of South China Normal University (SCNU) researcher Jiang Feng and his research group
Professor Lan Sheng’s Research Team Published a High-level Academic Paper in Nature Communications
Recently, the research team led by Prof Lan Sheng from the School of Information Optoelectronic Science and Engi
The School of Physics and Electronic Engineering publishes a high-level research paper on Quantum Science and Technology in Nature Photonics
The School of Physics and Electronic Engineering of SCNU has made important progress in quantum storage research:
Professor Ye Qi of the School of Mathematical Sciences, published a high-level Academic Paper in the Field of Applied Mathematics in Memoirs of the AMS
Recently, Prof Ye Qi, a new national talent recruited by the School of Mathematical Sciences of SCNU, and Prof